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BRL 3A 大鼠正常肝细胞

 发布时间:2017-01-17 点击量:250

BRL 3A   大鼠正常肝细胞说明书

General Information:

Organism:

Rattus norvegicus, rat

Tissue:

liver

Culture Properties:

adherent

Morphology:

fibroblast

 

Culture Method:

Complete Growth Medium:

DMEM (Gibco 12800-017)+10% FBS+ Penicillin/Streptomycin

Subculturing:

Volumes are given for a 25cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

  • Remove and discard culture medium.
  • Briefly rinse the cell layer with 0.25%(w/v) Trypsin-EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  • Add 1.0 to 2.0mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Discard Trypsin-EDTA solution.
  • Add 6.0 to 8.0mL of complete growth medium and aspirate cells by gently pipetting.
  • Add appropriate aliquots of the cell suspension to new culture vessels.
  • Incubate cultures at 37°C, 5% CO2.

Subcultivation Ratio:

A subcultivation ratio of 1:3 to 1:6 is recommended

Cryopreservation:

Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase